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Comstat Script, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sensitivity of QSI-treated P. aeruginosa biofilms to kanamycin. Confocal scanning laser microscopy (CLSM) photomicrographs of PAO1 biofilms grown in the presence or absence of QSI supernatant (5%). Three days later, the biofilm was exposed to 100 µg ml −1 kanamycin for 24 h. ( a ) No QSI supernatant or kanamycin; ( b ) QSI supernatant (5%); ( c ) 100 µg ml −1 kanamycin; ( d ) QSI supernatant (5%) plus 100 µg ml −1 kanamycin. Live cells were stained green and dead cells were stained red using a LIVE/DEAD Bac Light Bacterial Viability Kit. ( e ) <t>COMSTAT</t> quantification of (i) bio-volume, (ii) average thickness, and (iii) maximum thickness show that PAO1 forms thinner biofilms after being treated with kanamycin and QSI supernatant extract.
Comstat, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/comstat/product/MathWorks Inc
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Sensitivity of QSI-treated P. aeruginosa biofilms to kanamycin. Confocal scanning laser microscopy (CLSM) photomicrographs of PAO1 biofilms grown in the presence or absence of QSI supernatant (5%). Three days later, the biofilm was exposed to 100 µg ml −1 kanamycin for 24 h. ( a ) No QSI supernatant or kanamycin; ( b ) QSI supernatant (5%); ( c ) 100 µg ml −1 kanamycin; ( d ) QSI supernatant (5%) plus 100 µg ml −1 kanamycin. Live cells were stained green and dead cells were stained red using a LIVE/DEAD Bac Light Bacterial Viability Kit. ( e ) <t>COMSTAT</t> quantification of (i) bio-volume, (ii) average thickness, and (iii) maximum thickness show that PAO1 forms thinner biofilms after being treated with kanamycin and QSI supernatant extract.
Matlab 5.1, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sensitivity of QSI-treated P. aeruginosa biofilms to kanamycin. Confocal scanning laser microscopy (CLSM) photomicrographs of PAO1 biofilms grown in the presence or absence of QSI supernatant (5%). Three days later, the biofilm was exposed to 100 µg ml −1 kanamycin for 24 h. ( a ) No QSI supernatant or kanamycin; ( b ) QSI supernatant (5%); ( c ) 100 µg ml −1 kanamycin; ( d ) QSI supernatant (5%) plus 100 µg ml −1 kanamycin. Live cells were stained green and dead cells were stained red using a LIVE/DEAD Bac Light Bacterial Viability Kit. ( e ) <t>COMSTAT</t> quantification of (i) bio-volume, (ii) average thickness, and (iii) maximum thickness show that PAO1 forms thinner biofilms after being treated with kanamycin and QSI supernatant extract.
Image Processing Toolbox, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sensitivity of QSI-treated P. aeruginosa biofilms to kanamycin. Confocal scanning laser microscopy (CLSM) photomicrographs of PAO1 biofilms grown in the presence or absence of QSI supernatant (5%). Three days later, the biofilm was exposed to 100 µg ml −1 kanamycin for 24 h. ( a ) No QSI supernatant or kanamycin; ( b ) QSI supernatant (5%); ( c ) 100 µg ml −1 kanamycin; ( d ) QSI supernatant (5%) plus 100 µg ml −1 kanamycin. Live cells were stained green and dead cells were stained red using a LIVE/DEAD Bac Light Bacterial Viability Kit. ( e ) <t>COMSTAT</t> quantification of (i) bio-volume, (ii) average thickness, and (iii) maximum thickness show that PAO1 forms thinner biofilms after being treated with kanamycin and QSI supernatant extract.
Comstat2, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sensitivity of QSI-treated P. aeruginosa biofilms to kanamycin. Confocal scanning laser microscopy (CLSM) photomicrographs of PAO1 biofilms grown in the presence or absence of QSI supernatant (5%). Three days later, the biofilm was exposed to 100 µg ml −1 kanamycin for 24 h. ( a ) No QSI supernatant or kanamycin; ( b ) QSI supernatant (5%); ( c ) 100 µg ml −1 kanamycin; ( d ) QSI supernatant (5%) plus 100 µg ml −1 kanamycin. Live cells were stained green and dead cells were stained red using a LIVE/DEAD Bac Light Bacterial Viability Kit. ( e ) <t>COMSTAT</t> quantification of (i) bio-volume, (ii) average thickness, and (iii) maximum thickness show that PAO1 forms thinner biofilms after being treated with kanamycin and QSI supernatant extract.
Matlab 5 1, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sensitivity of QSI-treated P. aeruginosa biofilms to kanamycin. Confocal scanning laser microscopy (CLSM) photomicrographs of PAO1 biofilms grown in the presence or absence of QSI supernatant (5%). Three days later, the biofilm was exposed to 100 µg ml −1 kanamycin for 24 h. ( a ) No QSI supernatant or kanamycin; ( b ) QSI supernatant (5%); ( c ) 100 µg ml −1 kanamycin; ( d ) QSI supernatant (5%) plus 100 µg ml −1 kanamycin. Live cells were stained green and dead cells were stained red using a LIVE/DEAD Bac Light Bacterial Viability Kit. ( e ) <t>COMSTAT</t> quantification of (i) bio-volume, (ii) average thickness, and (iii) maximum thickness show that PAO1 forms thinner biofilms after being treated with kanamycin and QSI supernatant extract.
Matlab 5.3, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sensitivity of QSI-treated P. aeruginosa biofilms to kanamycin. Confocal scanning laser microscopy (CLSM) photomicrographs of PAO1 biofilms grown in the presence or absence of QSI supernatant (5%). Three days later, the biofilm was exposed to 100 µg ml −1 kanamycin for 24 h. ( a ) No QSI supernatant or kanamycin; ( b ) QSI supernatant (5%); ( c ) 100 µg ml −1 kanamycin; ( d ) QSI supernatant (5%) plus 100 µg ml −1 kanamycin. Live cells were stained green and dead cells were stained red using a LIVE/DEAD Bac Light Bacterial Viability Kit. ( e ) <t>COMSTAT</t> quantification of (i) bio-volume, (ii) average thickness, and (iii) maximum thickness show that PAO1 forms thinner biofilms after being treated with kanamycin and QSI supernatant extract.
Image Processing Tool, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sensitivity of QSI-treated P. aeruginosa biofilms to kanamycin. Confocal scanning laser microscopy (CLSM) photomicrographs of PAO1 biofilms grown in the presence or absence of QSI supernatant (5%). Three days later, the biofilm was exposed to 100 µg ml −1 kanamycin for 24 h. ( a ) No QSI supernatant or kanamycin; ( b ) QSI supernatant (5%); ( c ) 100 µg ml −1 kanamycin; ( d ) QSI supernatant (5%) plus 100 µg ml −1 kanamycin. Live cells were stained green and dead cells were stained red using a LIVE/DEAD Bac Light Bacterial Viability Kit. ( e ) COMSTAT quantification of (i) bio-volume, (ii) average thickness, and (iii) maximum thickness show that PAO1 forms thinner biofilms after being treated with kanamycin and QSI supernatant extract.

Journal: Royal Society Open Science

Article Title: Strain identification and quorum sensing inhibition characterization of marine-derived Rhizobium sp. NAO1

doi: 10.1098/rsos.170025

Figure Lengend Snippet: Sensitivity of QSI-treated P. aeruginosa biofilms to kanamycin. Confocal scanning laser microscopy (CLSM) photomicrographs of PAO1 biofilms grown in the presence or absence of QSI supernatant (5%). Three days later, the biofilm was exposed to 100 µg ml −1 kanamycin for 24 h. ( a ) No QSI supernatant or kanamycin; ( b ) QSI supernatant (5%); ( c ) 100 µg ml −1 kanamycin; ( d ) QSI supernatant (5%) plus 100 µg ml −1 kanamycin. Live cells were stained green and dead cells were stained red using a LIVE/DEAD Bac Light Bacterial Viability Kit. ( e ) COMSTAT quantification of (i) bio-volume, (ii) average thickness, and (iii) maximum thickness show that PAO1 forms thinner biofilms after being treated with kanamycin and QSI supernatant extract.

Article Snippet: COMSTAT was written as a script in MATLAB 5.1 and was equipped with Image Processing Toolbox.

Techniques: Microscopy, Staining