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Image Search Results
Journal: Royal Society Open Science
Article Title: Strain identification and quorum sensing inhibition characterization of marine-derived Rhizobium sp. NAO1
doi: 10.1098/rsos.170025
Figure Lengend Snippet: Sensitivity of QSI-treated P. aeruginosa biofilms to kanamycin. Confocal scanning laser microscopy (CLSM) photomicrographs of PAO1 biofilms grown in the presence or absence of QSI supernatant (5%). Three days later, the biofilm was exposed to 100 µg ml −1 kanamycin for 24 h. ( a ) No QSI supernatant or kanamycin; ( b ) QSI supernatant (5%); ( c ) 100 µg ml −1 kanamycin; ( d ) QSI supernatant (5%) plus 100 µg ml −1 kanamycin. Live cells were stained green and dead cells were stained red using a LIVE/DEAD Bac Light Bacterial Viability Kit. ( e ) COMSTAT quantification of (i) bio-volume, (ii) average thickness, and (iii) maximum thickness show that PAO1 forms thinner biofilms after being treated with kanamycin and QSI supernatant extract.
Article Snippet:
Techniques: Microscopy, Staining